When novel coronavirus pneumonia sweeps the globe, it is well known that the virus sampling tube is used routinely in our daily inspection and prevention. In many diseases, many infectious diseases are not obvious. Clinical experience alone is difficult to diagnose accurately and to use drugs accurately, which often leads to the illness.

For the collection of aerobic and anaerobic blood culture, straight needle vacuum blood collection is usually used, and the aerobic bottle should be the first bottle; If the butterfly wing needle is used for collection, the aerobic bottle should also be the first bottle. At this time, the air in the butterfly wing needle connecting pipe is introduced into the aerobic bottle; However, if the syringe is used for collection, the anaerobic bottle should be the first bottle, because when the blood in the syringe is injected into the blood culture bottle, the air bubbles will rise due to light air. At this time, there is a high possibility of gas in the blood at the upper part of the syringe, and the introduction of oxygen into the anaerobic bottle should be avoided.

The main component of the former is eagle's basic culture medium (MEM) or Hank's equilibrium salt, which is added with salts, amino acids, vitamins, glucose and proteins required for virus survival. This preservation solution uses phenol red sodium salt as an indicator. When the pH value of the solution is 6.6-8.0, the solution is pink. The preservation solution is added with necessary glucose, l-glutamine and protein. The protein is provided in the form of fetal bovine serum or bovine serum albumin, which can stabilize the protein shell of the virus.